Antimicrobial activity of chitosan nanoparticles loaded with 0.7% tetracycline against porphyromonas gingivalis

Abstract

Objectives: Nanoparticle technology has also come to the fore as a viable drug delivery strategy, providing opportunities for controlled release, protecting the active ingredient from enzymatic or environmental degradation, and local retention. Porphyromonas gingivalis is a Gram-negative obligate anaerobe rod involved in the etiology of periodontal disease. Tetracycline has been widely used as an adjuvant in periodontal therapy due to its antibacterial efficacy of these drugs. 
 Material and Methods: Samples of this study were the pure culture of P. gingivalis (ATCC 33 277) in Mueller Hinton Agar (MHA). The ionic gelation method made a Chitosan nanoparticle loaded with 0.7% tetracycline. Chitosan nanoparticles loaded with 0.7% tetracycline were directly transferred to the microbiology laboratory to maintain the stability of the material. Mueller Hinton Agar media inoculated with bacteria had holes to input chitosan nanoparticles loaded with 0.7% tetracycline. After repeating these operations four times, the incubator was incubated at 37 ° C for 48 hours. Calipers were used to observe and measure the diameter of the bright zone (clear zone) formed around the hole.
 Results: The result shows the potent antimicrobial effectiveness of chitosan nanoparticles loaded with 0,7% tetracycline with an inhibitory zone of 32.8 mm in vitro. 
 Conclusion: This research has shown a potent antimicrobial activity of chitosan nanoparticles loaded with 0,7% tetracycline against P. gingivalis in vitro.