Abstract
Methods Urine obtained from healthy volunteers was pooled and sterile filtered. Microdilution tests were performed with Escherichia coli ATCC 25922 in MHB and human urine. The pH of urine was adjusted to values ranging from 5 to 8. For simulating different glucose levels in urine of diabetic patients, urine was adjusted to glucose levels of 100 and 1000 mg/dL. Bacterial growth in different media was investigated by growth curves. Results obtained from MIC testing were confirmed by use of bacterial killing curves. Klebsiella oxytoca ATCC 700324 was used to investigate transferability of finding to other strains. Each experiment was performed 5 times.
Highlights
In vitro activity of antimicrobials is commonly assessed in well-characterized growth media like Mueller-Hinton broth (MHB); such media hardly reflect in vivo conditions
Strong impact on activity was observed at low pH values for fluoroquinolones, trimethoprim, amikacin and tigecycline. All these antimicrobials act intracellulary while impact of pH on cell membrane-active antibiotics like b-lactams and colistin was much lower
Mean ratios of MICs for E. coli at the 6 different conditions simulated in urine compared to MIC-values in standard MHB are presented
Summary
Antimicrobial activity of antibiotics in urine under different physiological conditions Angela Burian, Zeynep Erdogan, Mohammad Manafi, Markus Zeitlinger*. From 16th Scientific Symposium of the Austrian Pharmacological Society (APHAR) Vienna, Austria. From 16th Scientific Symposium of the Austrian Pharmacological Society (APHAR) Vienna, Austria. 25-27 November 2010
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