Antimicrobial Activity of AgNPs Synthesized Via Green Approach by Using Flowers of Bistortamacrophylla herb of Tungnath Himalaya Region

Abstract

Green synthesis of nano structured materials isan emerging field for the researchers working in the field of chemical, physical, life sciences, engineering and medical sciences, for the welfare of society.In the present study green synthesis of silver nanoparticles (AgNPs)was performed by using flowers of Bistorta macrophylla, a medicinal herb of polygonaceae family of Garhwal Himalayas. For green synthesis of AgNPs, the aqueous flowers extract and the aqueous solution of AgNO3(of 5 milli molar concentration) were well mixed in ratios of 1:9.The metal ion present in the solution reduced Ag+1 to Ag0 within three to four days, which was characterized by color change observation, UV, SEM-EDX, XRD, TEM etc. The absorption maxima of AgNPs of Bistorta macrophylla flowers (BMF) was obtained in the range of 451– 454nm for 5mM concentration of AgNO3, and the SEM images revealed that the average clustersize of synthesized Ag nanoparticles from 5BMF1:9ranges from 20 μm– 23μm, while the EDX analysis four elements show their presence as Ag (51.44 weight %), C (22.45 weight %), Cl (1.18 weight %) and O (22.24 weight%) in the synthesized AgNPs of 5mM(BMF1:9).XRD revealed that the crystals nature of synthesized AgNPs were cubic, and TEM results shows that the biosynthesized nanoparticles are agglomerated and irregular shaped in which size ranging from 12 nm to 38 nm. Synthesized AgNPs were also found to be active for potent antibacterial activity against various pathogenic and harmfull bacterial strains of humans like Pseudomonas Sp.(19.5mm), Bacillus cereus(21 mm) and CoNS (Coagulase Negative Staphylococci) (16.4 mm).Synthesized nanomaterial may be active for efficient clinical applications.