Antimicrobial Activity and Cytotoxic Effect on Gingival Cells of Silver Nanoparticles Obtained by Biosynthesis

Bogdan Andor,Delia Berceanu-Vaduva,Voichita Lazureanu,Alina Andreea Tischer (Tucuina),Adelina Cheveresan,Marioara Poenaru

doi:10.37358/rc.19.3.7006

Abstract

The emergence and development of biofilm is steadily increasing and its currently used prevention and control schemes are free of unwanted effects. Silver nanoparticles obtained through biosynthesis represent an area of interest in the biofilm approach. In the present study, silver nanoparticles were obtained by using the aqueous extract of rosemary leaves which were subsequently tested for antimicrobial activity and for the influence regarding viability of healthy gingival cells. Experiments have shown a significant antimicrobial activity on E. coli, S. aureus, P. aeruginosa and C. albicans. The viability of keratinocytes and gingival fibroblasts was influenced by higher concentrations of test compound (10 and 25 �g/mL).

Highlights

The emergence and development of biofilm is steadily increasing and its currently used prevention and control schemes are free of unwanted effects
Green silver nanoparticles were evaluated by disk diffusion (DD) method for Antimicrobial activity (AMA) against Staphylococcus aureus (+, ATCC 25923), Escherichia coli (, ATCC 25922), Pseudomonas aeruginosa (, ATCC 27853), and Candida albicans
The RAE_AgNPs were characterized by well-known methods, and the data regarding their characteristics are presented in table 1

Summary

Introduction

The emergence and development of biofilm is steadily increasing and its currently used prevention and control schemes are free of unwanted effects. Silver nanoparticles exert significant antibacterial and antiviral activities, being able to interact with different microorganisms, thereby reducing the growth and maturation of biofilm [11]. There are studies regarding the toxicity of silver nanoparticles and samples with a spherical shape, and size between 10-50 nm are presented as a safe profile for healthy cells in terms of viability and in terms of acute/subacute toxicity on an animal model no denotive changes regarding health status after i.p. administration of low doses was observed [12].

Results

Conclusion