Abstract

Article history: Received on: 12/06/2015 Revised on: 17/07/2015 Accepted on: 11/08/2015 Available online: 27/09/2015 The essential oil and hydrosol extract of Nepeta nepetella subsp. amethystina, obtained by hydrodistillation and analysed by GC, GC–MS, 1H and 13C NMR, were evaluated for their antimicrobial activity using disks diffusion method and MICs against thirteen pathogenic microorganisms. The chemical analysis of essential oil and hydrosol extract allowed the identification of 34 and 12 constituents representing 96.77% and 80.07% of total oil and hydrosol extract, respectively. The two isomers 4a,7,7a-nepetalactone and 4a, 7, 7a-nepetalactone were found to be the major components in N. amethystina essential oil and hydrosol extract, , 7-nepetalactone and 4a,7a,7respectively. The two isomers 4a -nepetalactone were found to be the major components in N.7a amethystina essential oil and hydrosol extract. Candida albicans strains were the most sensitive microorganisms towards N. amethystina essential oil and hydrosol extract, which have the largest inhibition zones (˃30mm) and the lower MICs values (<1.25%). The anti-mold activity, carried out by the diffusion method, showed that N. amethystina essential oil has a very interesting activity against Aspergillus flavus and Cladosporium herbarium.

Highlights

  • The genus Nepeta which belonged to Lamiaceae family is represented by more than 250 species distributed mainly in Africa, Europe and Asia (Mabberly, 1997). 7% of total Nepeta species growth wild in the Iberian Peninsula and North Africa, in which only five species exist in Algeria (Quézel and Santa, 1962; Greuter et al, 1986)

  • The chemical composition of essential oil (EO) and hydrosol extract (HY) from N. amethystina was listed in table 1

  • A total of thirty-four compounds were identified in N. amethystina EO and HY obtained from the aerial parts of N. amethystina, representing 96.77 and 80.07 % respectively

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Summary

Introduction

The genus Nepeta which belonged to Lamiaceae family is represented by more than 250 species distributed mainly in Africa, Europe and Asia (Mabberly, 1997). 7% of total Nepeta species growth wild in the Iberian Peninsula and North Africa, in which only five species exist in Algeria (Quézel and Santa, 1962; Greuter et al, 1986). The genus Nepeta which belonged to Lamiaceae family is represented by more than 250 species distributed mainly in Africa, Europe and Asia (Mabberly, 1997). 7% of total Nepeta species growth wild in the Iberian Peninsula and North Africa, in which only five species exist in Algeria (Quézel and Santa, 1962; Greuter et al, 1986). According to taxonomic revision of Ubera & Valdes (Castrillón and Jiménez, 1983), N. amethystina is a subspecies of N. nepetella. This medicinal plant is found in Saharan Atlas in dry stony places. The essential oils of some Nepeta species have been extensively studied, which are mainly characterized by nepetalactone (Dabiri and Sefidkon, 2003; Sefidkon et al, 2006).

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