Abstract
The evaluation of antimicrobial study of few medicinally used plants to control the growth of some pathogenic microorganisms. The standard protocols with slight modifications were utilized to prepare silver nanoparticles using five medicinal plants extracts namely, Juniperus exclsa, Moringa oleifera, Prosopis juliflora, Pithecellobium dulce and Eucalyptus globulus, were randomly collected from Jeddah and Taif city, Kingdom of Saudi Arabia. The formation of nanoparticles was observed by UV/Vis spectra and further characterization has been carried out by different analytical techniques. The shape, size and morphology of nanoparticles were observed by SEM and TEM images. The prepared extracts and nanoparticles were tested for antimicrobial study and the total phenolics of the methanolic extract was confirmed by GCMS and HPLC techniques. The nanoparticles were assessed against five microorganisms such as Gram-positive Staphylococcus aureus ATCC 6538, Gram-negative Pseudomonas aeruginosa ATCC 9027 and Escherichia coli ATCC 8739, yeast Candida albicans ATCC 10231and fungi Aspergillus niger ATCC 16404.
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