Abstract
Aim of present research was to evaluate the anti-bacterial, anti-fungal, anti-oxidant and multi-stress activities of a medicinal plant Mint Lemonade plant extract. Fresh mint was purchased from the nearest market. The samples were cleaned with distilled led water and its leaves, stem and roots were separated them all. The material was dried in shade at room temperature (24ºC). The three types of extracts were prepared for each sample of mint plantviz, ethanol, n-hexane and water. The antibacterial and antifungal activities of ethanolic, n-haxene and aqueous mint extract was determined on Escherichia coli, Bacillus cereus, Pseudomonas, Aeromonas hydrophila, Aspergillus niger, Aspergillus flavous and Rhizopus stolonifer respectively. From the results of the antibacterial and antifungal activities it was clear that the aqueous extract showed no inhibitory effect on test these microorganisms. In ethanolic extract of antibacterial activity Escherichia coli showed the maximum zone of inhibition with diameters of 3.90 cm at 50 µl dose. Bacilluscereus showed the zone of inhibition diameter 4.9 cm. Pseudomonas showed the maximum zone of inhibition in diameter 3.86mm.Aeromonas hydrophila expressed the maximum zone of inhibition in diameter 3.86mm. The n-hexane extracts antibacterial activity of E. coli, Bacillus cereus, Pseudomonas and Aeromonas hydrophila showed the zone of inhibition 3.86 cm, 4.5 cm, 7.90 mm and 4.71 cm respectively, while the positive control showed inhibition zone about 0.0 cm. The antifungal activity in n-haxene extract showed the maximum result in Aspergillus flavous 18 cm of the area of inhibition. In ethanol extracts antifungal activity showed the maximum result in Aspergillus flavous 4.8 cm of the zone of inhibition. The inhibitory action of mint plant extracts indicated that the plant may be used as potential antibacterial agent.
Highlights
Most of medicinal and aromatic plants contain secondary metabolites which play vital role in the protection of the plants as antiviral, antibacterial, insecticides and antifungal
Complete research effort was completed at the chemical biology and microbiology research laboratory at the institute of Molecular Biology and Biotechnology (IMBB) and plants Biotechnology Laboratory of Centre of research in Molecular Medicine (CRIMM), the University of Lahore
The sample (Pseudomonas aeuginosa, Bacillus subtilis and Escherichia coli) of these organisms were spread on freshly Nutrient Agar medium in Petri dishes and these plates were positioned in incubator for 24 hours
Summary
Most of medicinal and aromatic plants contain secondary metabolites which play vital role in the protection of the plants as antiviral, antibacterial, insecticides and antifungal. Essential oils extracted from these plants are complex of natural compounds that reduces foodborne pathogens and decrease the use of synthetic and semisynthetic antimicrobial compounds [1] due to their side effects such as carcinogenesis [2] Herbal compounds such as phenolic compounds that are found in these plants act as antioxidant and radical scavengers [3,4,5,6]. Citrus genus is widely used fruit crop due to its natural antioxidant properties [23] Phenolic compounds such as essential oils, dietary fiber, carotenoids, vitamins and minerals along with ascorbic acid, are the most important elements of citrus which contributes to its healthy properties [24,25]. The present study was conducted to success the antimicrobial activities of mint plant extracts from plants grown under salt and heavy metal stress for evaluation of differences generated after stress of salt and heavy metals
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