Abstract
Ganoderma is a well-known genus of medicinal mushrooms. The biological activity of the fruiting bodies of G. mbrekobenum (previously identified as Ganoderma sp. EGDA, (AC: LN774971) is scarcely studied. The microorganisms including bacteria and fungi were chosen for screening of the antimicrobial activity produced by G. mbrekobenum strain EGDA. The bioactive compounds were extracted from aqueous, petroleum ether, chloroform, ethyl acetate, and methanol extracts. The higher antibacterial activity produced by methanol extract was against Bacillus subtilis and B. cereus (14.13 ± 0.12 mm, 13.03 ± 0.12 mm, respectively). Water fraction showed antibacterial effect against most of the test bacterial strains. The highest antifungal activity produced by methanol extract was against Fusarium oxysporum I and F. oxysporum f. sp. lycopersici (16.37 ± 0.03 mm 15.67 ± 0.19 mm, respectively). Gas chromatography/mass spectrometry analysis of the separated fractions revealed the identification of 46 compounds.
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