Antimicrobial action of photodynamic therapy in root canals using LED curing light, curcumin and carbopol gel.

Abstract

To evaluate the capacity of carbopol gel to maintain the intensity of a LED curing light (blueLED) along the length of prepared root canals in bovine teeth, and to assess the antimicrobial capacity of curcumin photoactivated by a LED curing light in the presence of carbopol gel. Experiment 1: Eight straight roots of bovine incisors were standardized to a length of 15mm, and the root canals instrumented up to a size 120K-file. The LED curing light was irradiated inside the root canals using an aluminium collimator (1.5mm in diameter) placed at the orifice (n=8). Initially, the irradiation was performed in empty root canals and then repeated with the root canals filled with carbopol gel. Simple standardized photographs of the roots were taken with a digital camera in the mesial perspective during the irradiation procedure and the images analysed in OriginLab software to verify the light intensity along the length of the root. Experiment 2: Twenty dentine blocks were obtained from the cervical third of bovine incisors using a trephine bur. Biofilms were induced for 21days on the blocks using Enterococcus faecalis (ATCC 4083) at 109 cellsmL-1 . The blocks were treated according to the groups (n=5): positive control; standard PDT (methylene blue+diode Laser); curcumin; LED curing light; and curcumin+LED curing light. After the treatment, the samples were dyed with Live/Dead BacLight Bacterial Viability solution and fluorescence images were obtained by Confocal Scanning Laser Microscopy (CSLM). Experiment 3: Thirty-two roots of bovine incisors were prepared as described in experiment 1. Their dentinal tubules were contaminated and the root canals treated according to the groups (n=8): positive control; standard PDT; curcumin+LED curing light; curcumin+carbopol gel+LED curing light. The specimens were sectioned longitudinally and the split roots were treated with the Live/Dead dye to obtain fluorescence images by CSLM. All images were processed using BioImageL software to measure the percentage of viable bacteria and the data analysed statistically using the nonparametric Kruskal-Wallis test (α<0.05). In Experiment 1, carbopol gel did not improve the intensity of LED light transmission along the root canal. In Experiment 2, a significant decrease (P<0.05) in bacterial viability occurred in the following order: positive control<only LED curing light<only curcumin<curcumin+LED curing light=standard PDT; and in Experiment 3 positive control=curcumin+LED curing light≤curcumin+gel+LED curing light≤standard PDT. Similar disinfection effectiveness was obtained using curcumin+LED curing light and methylene blue+660nm LASER (standard PDT). The use of carbopol gel did not favour a greater transmission of LED light along the root canal and also resulted in less bacterial killing when used in endodontic PDT.