Antimicrobial action of four herbal plants over mixed-species biofilms of Candida albicans with four different microorganisms.

Abstract

This study aimed to evaluate the antimicrobial effect of four herbal plants glycolic extracts over mixed-species biofilm composed of Candida albicans (C.albicans) and another pathogenic bacterium as alternative therapy to be investigated. Four plants extract of Pfaffia paniculata roots; Hamamelis virginiana leaf, Stryphnodendron barbatiman tree bark and Gymnema sylvestre stem and leaves were tested over multi-species biofilm of C.albicans (ATCC 18804) and Streptococcus mutans (ATCC 35688), Staphylococcus aureus (ATCC 6538), Enterococcus faecalis (ATCC 4083) or Pseudomonas aeruginosa (ATCC 15442) for 5min and 24h and colony forming units per millilitre was calculated. The data were analysed using Kruskal-Wallis with Dunn's test (p≤ 0.05). All tested extracts showed antimicrobial action over the mixed-species biofilms after 24h. Some extracts eliminated totally the biofilms. The glycolic extract of P.paniculata, H.virginiana, S.barbatiman and G.sylvestre are effective over mixed-species biofilms and may be indicated as endodontic irrigant or intracanal medication.