Abstract
Effective antimicrobial preparations, other than antibiotics, are important for the treatment of potentially fatal drug-resistant infections. Methicillin-resistant Staphylococcus aureus (MRSA) is one of the leading causes of hospital-acquired and post- operative infections. Fortunately, the antimicrobial properties of platelet-rich plasma (PRP) against various microorganisms enable its potential use as an alternative to conventional antibiotics. The present work was designed to evaluate the hypothesized antimicrobial activity of PRP against MRSA infected skin wounds. Six adult male dogs were divided equally into control and PRP groups. Unilateral circular full-thickness skin wounds were created then a MRSA suspension was injected locally. Treatment started at 1st week post infection with subcutaneous infiltration of autologous activated PRP every week in the PRP group and with topical application of clindamycin cream twice daily in the control group. PRP decreased wound size and significantly increased wound contractility and re-epithelization, as confirmed by histopathological and immunohistochemical findings. Also PRP treated group showed significant decrease in ROS and redox imbalance with over expression of the TNF-α and VEGFA genes that indicate angiogenesis and maximum antibacterial activity after three weeks. In conclusion, CaCl2-activated PRP exhibited antimicrobial activity against MRSA infection, which improved the infected wound healing re-epithelization and granulation tissue formation.
Highlights
Wound infections with multidrug-resistant strains of pathogenic microorganisms are significant global health problems
Platelets secrete at least seven growth factors that are essential in the initiation of the healing process, including the platelet-derived growth factor (PDGF) isomers PDGFaa, PDGFab and PDGFbb; transforming growth factor (TGF)-b1, TGF-b2, vascular endothelial growth factor (VEGF), and epithelial growth factor (EGF)[11]
The treatment superiority of Platelet-rich plasma (PRP) depends on high concentrations of platelets in concentrated plasma that harbour a wide variety of growth factors[35]
Summary
Wound infections with multidrug-resistant strains of pathogenic microorganisms are significant global health problems. After skin injury, platelet aggregation is induced to form a fibrin clot that supports haemostasis and recruits several cell types to the wound[5]. These cellular activities are mediated by cytokines and growth factors[6]. Platelets secrete at least seven growth factors that are essential in the initiation of the healing process, including the platelet-derived growth factor (PDGF) isomers PDGFaa, PDGFab and PDGFbb; transforming growth factor (TGF)-b1, TGF-b2, vascular endothelial growth factor (VEGF), and epithelial growth factor (EGF)[11] These growth factors promote rapid increases in the numbers of undifferentiated mesenchymal cells at the wound site during repair and healing. PL is a complex mixture of plasma components whose influences have not yet been studied in detail
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
