Anti-metastasis activity of black rice anthocyanins against breast cancer: analyses using an ErbB2 positive breast cancer cell line and tumoral xenograft model.

Qiang Zou,Xiao-Ping Yu,Jie Zhou,Yan-Feng Zhu,Xiao-Li Peng,Xiang-Yan Chen,Sui-Yan Li,Bin Han,Wei Chen,Li-Ping Luo

doi:10.7314/apjcp.2014.15.15.6219

Qiang Zou, Xiao-Ping Yu + Show 8 more

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https://doi.org/10.7314/apjcp.2014.15.15.6219

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Abstract

Increasing evidence from animal, epidemiological and clinical investigations suggest that dietary anthocyanins have potential to prevent chronic diseases, including cancers. It is also noteworthy that human epidermal growth factor receptor 2 (ErbB2) protein overexpression or ErbB2 gene amplification has been included as an indicator for metastasis and higher risk of recurrence for breast cancer. The present experiments investigated the anti-metastasis effects of black rice anthocyanins (BRACs) on ErbB2 positive breast cancer cells in vivo and in vitro. Oral administration of BRACs (150 mg/kg/day) reduced transplanted tumor growth, inhibited pulmonary metastasis, and decreased lung tumor nodules in BALB/c nude mice bearing ErbB2 positive breast cancer cell MDA-MB-453 xenografts. The capacity for migration, adhesion, motility and invasion was also inhibited by BRACs in MDA-MB-453 cells in a concentration dependent manner, accompanied by decreased activity of a transfer promoting factor, urokinase-type plasminogen activator (u-PA). Together, our results indicated that BRACs possess anti-metastasis potential against ErbB2 positive human breast cancer cells in vivo and in vitro through inhibition of metastasis promoting molecules.

Highlights

Breast cancer is the most frequently diagnosed malignancy and the leading global cause of cancer motility in women, accounting for 23% of cancer diagnoses (1.38 million) and 14% of cancer deaths (458 000) each year (DeSantis et al, 2011; Jemal et al, 2011)
Together, our results indicated that black rice anthocyanins (BRACs) possess anti-metastasis potential against ErbB2 positive human breast cancer cells in vivo and in vitro through inhibition of metastasis promoting molecules
The MDA-MB-453 xenografts were established on BALB/c nude mice. (A) The anesthetic mice of control and BRACs treatment groups and the isolated primary tumors by day 42. (B) Body weights of mice were measured every five days from the day BRACs treatment started. (C) Tumor volumes were measured and calculated every three days

Summary

Introduction

Breast cancer is the most frequently diagnosed malignancy and the leading global cause of cancer motility in women, accounting for 23% of cancer diagnoses (1.38 million) and 14% of cancer deaths (458 000) each year (DeSantis et al, 2011; Jemal et al, 2011). It is noteworthy that human epidermal growth factor receptor 2 (ErbB2) protein overexpression or ErbB2 gene amplification has been included as an indicator for metastasis and higher risk of recurrence for breast cancer. Materials and Methods: The present experiments investigated the anti-metastasis effects of black rice anthocyanins (BRACs) on ErbB2 positive breast cancer cells in vivo and in vitro. Results: Oral administration of BRACs (150 mg/kg/day) reduced transplanted tumor growth, inhibited pulmonary metastasis, and decreased lung tumor nodules in BALB/c nude mice bearing ErbB2 positive breast cancer cell MDA-MB-453 xenografts. Conclusions: Together, our results indicated that BRACs possess anti-metastasis potential against ErbB2 positive human breast cancer cells in vivo and in vitro through inhibition of metastasis promoting molecules

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