Abstract
BackgroundThe search for new antimalarial drugs has become increasingly urgent due to plasmodial resistance to existing drugs. As part of this global effort, the present study aimed at evaluating the antimalarial activity of two traditionally used medicinal plants against the disease.MethodsAcute toxicity and four-day suppressive effects of aqueous, methanol and chloroform extracts of the seed and leaf of Brucea antidysenterica and Ocimum lamiifolium, respectively, were investigated in Swiss albino mice using Plasmodium berghei using standard procedures.ResultsMethanol extract of the leaves of O. lamiifolium did not exhibit any sign of acute toxicity up to the dose of 2000 mg/kg body weight. However, all mice provided with seeds of B. antidesenterica at a dose of 2000 mg/kg body died within 24 h. The aqueous, methanol and chloroform crude extracts of B. antidesenterica significantly (p < 0.05) inhibited parasitaemia in a dose-dependent manner and prevented body weight loss at doses of 200, 400 and 600 mg/kg body weight. In addition, the extracts prolonged the mean survival time of P. berghei-infected mice compared to the non-treated control. However, it did not prevent reduction in packed cell volume except the chloroform extract in three doses and methanol extract at 200 mg/kg and 400 mg/kg. Extracts from O. lamiifolium also exhibited significant (p < 0.05) antiplasmodial activities. The extracts did not prevent body weight loss and PCV reduction, especially in chloroform. The highest suppression was recorded from aqueous crude extract of O. lamiifolium with 35.53 % in the dose of 600 mg/kg. On the other hand, a similar higher suppression was found in both methanol and chloroform of crude extracts of B. antidesenterica with 47.70 %, 46.44 % of chemosuppression, respectively, in its highest dose tested.ConclusionCrude aqueous, methanol and chloroform extracts of the two medicinal plants possess acceptable antimalarial effects. However, further investigation should be pursued on toxicity study and to isolate the bioactive components responsible for the observed antimalarial action of the plants.
Highlights
The search for new antimalarial drugs has become increasingly urgent due to plasmodial resistance to existing drugs
Study shows that the organic leaf extract of Ocimum gratissimum, a species found under the genus where Ocimum lamiifolium belongs, suppressed parasitaemia of Plasmodium berghei in mice by 88.07 % at a dose of 100 mg/Kg body weight [14]
Extraction yields of plant materials For seeds of Brucea antidysenterica, the highest yield was obtained for their methanol extract (8.69 g), followed by that for aqueous (5.64 g) and chloroform (4.79 g) extracts
Summary
The search for new antimalarial drugs has become increasingly urgent due to plasmodial resistance to existing drugs As part of this global effort, the present study aimed at evaluating the antimalarial activity of two traditionally used medicinal plants against the disease. In vivo and in vitro studies conducted on fruit extracts of Brucea javanica, a species closely related to Brucea antidysenterica, demonstrated antiplasmodial activity of the plant attributable to quassinoid constituents [13]. Study shows that the organic leaf extract of Ocimum gratissimum, a species found under the genus where Ocimum lamiifolium belongs, suppressed parasitaemia of Plasmodium berghei in mice by 88.07 % at a dose of 100 mg/Kg body weight [14]. The present study, aimed at assessing the antimalarial activity of the crude extracts of the seed of B. antidysenterica and leaf of O. lamifolium against P. berghei in Swiss albino mice
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