Anti-leptospiral potential of Zingiber zerumbet (L.) Smith crude extracts against Leptospira spp.

Abstract

Event Abstract Back to Event Anti-leptospiral potential of Zingiber zerumbet (L.) Smith crude extracts against Leptospira spp. Asmah Hamid1*, Nurul A. Abdul Aziz1, Latifah Ibrahim2, Farah W. Ibrahim1 and Nurul F. Jufri1 1 School of Diagnostic and Applied Health Sciences, Faculty of Health Sciences, National University of Malaysia, Malaysia 2 Institute for Medical Research, Ministry of Health (Malasiya), Malaysia Background Leptospirosis is one of the emerging infectious diseases that the number of cases had been increasing progressively in Malaysia. Numerous attempts have been made to control the disease by using chemoprophylaxis but only showed limited success. The present study investigated the anti-leptospiral potential of hexane, ethyl acetate and methanol extracts of Zingiber zerumbet. Methods The extracts were assayed for anti-leptospiral activity using broth microdilution method towards Leptospira interrogans (serovar Batavie, Canicola, Australis) and Leptospira biflexa serovar Patoc. The DNA damaging properties of Z. zerumbet on DNA of Leptospira sp. was assessed by incubating the DNA of Leptospira sp. in the presence or absence of Z. zerumbet crude extracts and subsequently analyzed by electrophoresis. Results The anti-leptospiral activity of Z. zerumbet hexane extracts showed an IC₅₀ value of 248 μg/ml towards L. interrogans serovar Canicola, IC₅₀ value 125 μg/ml towards L. interrogans serovar Australis, IC₂₅ value 15.63 μg/ml towards L. interrogans serovar Batavie and IC₅₀ value 109 μg/ml towards L. biflexa serovar Patoc. However, ethyl acetate and methanol extracts exhibited a weak/mild anti-leptospiral activity. Since the hexane extract of Z. zerumbet displayed strong anti-leptospiral activity, the DNA damaging properties of this extract was tested at their IC₅₀ and IC25 values that were specific to each serovars. The result showed that hexane extract do not possess DNA damaging properties towards L. interrogans serovar Canicola and Bataviae as there was no smearing or fragmentation of DNA band. For L. biflexa serovar Patoc and L. interrogans serovar Australis there were DNA damage which was evidenced by the appearance of fragmented DNA on agarose gel. Conclusion The present study showed that Z. zerumbet hexane extract has the potential to inhibit the growth of Leptospira spp. by damaging the bacterial DNA. Keywords: Anti-leptospiral, Zingiber zerumbet, Leptospira, DNA Damage, extract Conference: International Conference on Drug Discovery and Translational Medicine 2018 (ICDDTM '18) “Seizing Opportunities and Addressing Challenges of Precision Medicine”, Putrajaya, Malaysia, 3 Dec - 5 Feb, 2019. Presentation Type: Poster Presentation Topic: Infectious diseases Citation: Hamid A, Abdul Aziz NA, Ibrahim L, Ibrahim FW and Jufri NF (2019). Anti-leptospiral potential of Zingiber zerumbet (L.) Smith crude extracts against Leptospira spp.. Front. Pharmacol. Conference Abstract: International Conference on Drug Discovery and Translational Medicine 2018 (ICDDTM '18) “Seizing Opportunities and Addressing Challenges of Precision Medicine”. doi: 10.3389/conf.fphar.2018.63.00117 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 30 Sep 2018; Published Online: 17 Jan 2019. * Correspondence: Dr. Asmah Hamid, School of Diagnostic and Applied Health Sciences, Faculty of Health Sciences, National University of Malaysia, Kuala Lumpur, Malaysia, asmah0901@ukm.edu.my Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Asmah Hamid Nurul A Abdul Aziz Latifah Ibrahim Farah W Ibrahim Nurul F Jufri Google Asmah Hamid Nurul A Abdul Aziz Latifah Ibrahim Farah W Ibrahim Nurul F Jufri Google Scholar Asmah Hamid Nurul A Abdul Aziz Latifah Ibrahim Farah W Ibrahim Nurul F Jufri PubMed Asmah Hamid Nurul A Abdul Aziz Latifah Ibrahim Farah W Ibrahim Nurul F Jufri Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.