Abstract
Cinnamomum camphora (Linn.) Presl has been widely used in traditional Chinese medicine for a variety of purposes. Our previous study indicated the antibacterial mechanism of the essential oil (EO) from C. camphora leaves; however, its anti-inflammatory activity and the underlying mechanism have not been clearly demonstrated. Thus, the present study investigated its anti-inflammatory property. Our data revealed that EO significantly decreased the release of nitric oxide (NO) and the mRNA expression of inducible NO synthase (iNOS) in lipopolysaccharide (LPS)-induced BV2 microglial cells. EO also attenuated LPS-induced increase in the mRNA expression and secretion of inflammatory cytokines including interleukin-6 (IL-6), IL-18, IL-1β and tumor necrosis factor-α (TNF-α). Furthermore, the metabolic profiles of LPS-induced BV2 microglial cells treated with or without EO were explored. Thirty-nine metabolites were identified with significantly different contents, including 21 upregulated and 18 downregulated ones. Five pathways were enriched by shared differential metabolites. Compared with the control cells, the glucose level was decreased, while the lactate level was increased, in the culture supernatant from LPS-stimulated cells, which were reversed by EO treatment. Moreover, compared to the LPS-treated group, the activities of phosphofructokinase (PFK) and pyruvate kinase (PK) in EO group were decreased. In summary, the current study demonstrated that EO from C. camphora leaves acts as an anti-inflammatory agent, which might be mediated through attenuating the glycolysis capacity of microglial cells.
Highlights
Cinnamomum camphora (Linn.) Presl, commonly known as camphor tree or camphor laurel, is widely cultivated in Southern China as an ornamental plant and a material for wood furniture [1].In traditional Chinese medicine, the essential oil from this plant (EO) has been widely used to treat inflammation, rheumatic conditions, and muscular strains [2]
The BV2 microglial cells were treated with EO at concentrations ranging of 7.8-250 μg/mL for 48 h
The results showed that EO did not obviously affect cell viability even at a concentration up to 250 μg/mL (Figure 1A)
Summary
Cinnamomum camphora (Linn.) Presl, commonly known as camphor tree or camphor laurel, is widely cultivated in Southern China as an ornamental plant and a material for wood furniture [1].In traditional Chinese medicine, the essential oil from this plant (EO) has been widely used to treat inflammation, rheumatic conditions, and muscular strains [2]. Inflammation is self-protective responses of body tissues to harmful stimuli, such as pathogens, damaged cells, or irritants [13]. Dysregulated inflammation, such as long-term chronic inflammation, has been considered to play key roles in the onset and development of metabolic disorders, neurodegeneration, and cardiovascular diseases [13,14]. Many studies investigated that microglia-mediated neuroinflammation plays an essential role in the pathophysiological process of multiple neurological disorders [15,16,17]. It is of significance to use BV2 microglia cells to study the anti-inflammatory effect of EO
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
