Abstract
This study reports the anti-inflammatory activity-guided fractionation of the aerial part of Piper bavinum C. CD. (Piperaceae) that led to the isolation of eight secondary metabolites (1–8). The chemical structures of 1–8 were established mainly by NMR and mass spectra. Compound 5 was isolated from P. bavinum for the first time. All the isolated compounds were evaluated against LPS-induced NO production in macrophage RAW 264.7 cells in vitro. Among them, compound 4 showed the most potent inhibitory activity against the LPS-induced NO production with an IC50 value of 5.2 μM followed by compound 5 that inhibited NO production with an IC50 value of 13.5 μM. In the protein levels, compound 4 suppressed LPS-induced COX-2 and iNOS expressions in a dose-dependent manner. The results suggested that P. bavinum and its constituents might exert anti-inflammatory effects.
Highlights
Inflammation is a protective response that occurs following trauma, infection, or tissue injury [1]
nitric oxide synthases (NOSs) included inducible nitric oxide synthase that is extremely expressed in macrophage cells, and the activation of iNOS generally leads to some autoimmune diseases [2]
During a screening program to discover inflammation inhibitors from natural sources, we have found that the n-hexane and ethyl acetate (EtOAc) fractions of Vietnamese P. bavinum exhibited appreciable inhibitory activity in LPS-induced NO production in macrophage RAW 264.7 cells
Summary
Viet Dung Hoang, Phi Hung Nguyen, Minh Thu Doan, Manh Hung Tran, Nhu Tuan Huynh, Huu Tung Nguyen ,5,6 Byung Sun Min, and Dao Cuong To 5,6. Is study reports the anti-inflammatory activity-guided fractionation of the aerial part of Piper bavinum C. (Piperaceae) that led to the isolation of eight secondary metabolites (1–8). Compound 5 was isolated from P. bavinum for the first time. All the isolated compounds were evaluated against LPS-induced NO production in macrophage RAW 264.7 cells in vitro. Compound 4 showed the most potent inhibitory activity against the LPS-induced NO production with an IC50 value of 5.2 μM followed by compound 5 that inhibited NO production with an IC50 value of 13.5 μM. Compound 4 suppressed LPS-induced COX-2 and iNOS expressions in a dose-dependent manner. E results suggested that P. bavinum and its constituents might exert anti-inflammatory effects Compound 4 suppressed LPS-induced COX-2 and iNOS expressions in a dose-dependent manner. e results suggested that P. bavinum and its constituents might exert anti-inflammatory effects
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