Anti-Inflammatory and Gastroprotective Activities of C udrania Tricuspidata Leaf Extract Against Acute HCl/Ethanol-Induced Gastric Mucosal Injury in Sprague-Dawley Rats

Abstract

We investigated the inhibitory effect of Cudrania tricuspidata ethanol 10% extract (CTL10) on gastric inflammation induced by acute ethanol treatment in Sprague-Dawley rats. After oral administration of CTL10 (100 or 300 mg/kg) for 7 days, acute gastric inflammation was induced by 70% ethanol and 0.15 M HCl. After 1 h of ethanol administration, the animals were sacrificed. Pretreatment with CTL10 showed attenuation of gastric mucosal injury, hemorrhages and gastric juice secretion induced by ethanol administration. Oral administration of CTL10 significantly decreased the levels of lipid peroxidation and increased superoxide dismutase activity. Additionally, pretreatment with 300 mg/kg CTL10 significantly decreased the expression of nuclear factor-κB, cyclooxygenase-2, interferon-γ, interleukin-6 and tumor necrosis factor-α compared with the gastric inflammation group. Based on this study, CTL10 may be considered a potential agent to control acute gastric inflammation induced by alcohol through the antioxidative effect of CTL10. Practical Applications Ethanol directly and dose-dependently impairs the gastric mucosal barrier, and the molecular mechanisms underlying ethanol-induced gastric inflammation remain incompletely understood. However, there is evidence that free radical production and oxidative stress play a major role in the pathogenesis of acute gastric inflammation by ethanol. We investigated the inhibitory effect of Cudrania tricuspidata ethanol 10% extract (CTL10) on gastric inflammation induced by acute ethanol treatment in Sprague-Dawley (SD) rats. We found that CTL10 has inhibitory effects on gastric inflammation induced by acute ethanol treatment in SD rats. Although the exact mechanism underlying these effects is unclear, the effects on acute gastric inflammation suggest a mechanism involving the antioxidant properties of C. tricuspidata. C. tricuspidata may be a new alternative for the clinical management of gastric inflammation while serving as an antioxidant against oxidative stress.