Abstract
Objective: The present study aims to investigate anti-inflammatory, analgesic and antioxidant effects of the hydroacetonic extract (HAE) from Wissadula amplissima whole plant and to determine its polyphenol and flavonoid contents.Methods: Extraction was achieved by soaking defatted W. amplissima with aqueous acetone solvent. Acute toxicity and pharmacological investigations were performed per os and intraperitoneally on Naval Medical Research Institute (NMRI) mice. The carrageenan-induced paw edema and the acetic acid-induced writhing tests were used to assess the in vivo anti-inflammatory and analgesic activities. Inhibition of xanthine oxidase and lipoxygenase were carried out in vitro using endpoint spectrophotometric assays at 295 nm and 234 nm. 2,2-diphenyl-1-picrylhydrazyl (DPPH), Ferric Reducing Antioxidant Power (FRAP), lipid peroxidation and desoxyribose degradation assays were used to point out antioxidant activities of extract. Aluminium chloride colorimetric method was used to determine total flavonoids content when and total phenolic content was measured by the method Folin-ciocalteu reagent.Results: W. amplissima’s HAE exhibited a slight acute toxicity with no lethality observed. Significant (p<0.05) anti-inflammatory and analgesic activities were recorded as well as strong inhibitory activities on lipoxygenase and xanthine oxidase. W. amplissima’s HAE also demonstrated interesting antioxidant activities particularly in preventing lipid peroxidation. Polyphenol and flavonoid contents were respectively estimated to 64.33±1.34 mg Gallic Acid Equivalent (GAE) and 35.17±0.77 mg Quercetin Equivalent (QE) per 100 mg of W. amplissima’s HAE.Conclusion: HAE of W. amplissima exhibited a promising anti-inflammatory and analgesic potential compared to the reference (acetylsalicylic acid, Hydrocortisone, paracetamol) and regarding the inhibition of lipoxygenase and lipid peroxidation. The anti-inflammatory and analgesic activities seem to be related to the antioxidant and enzymes inhibitors compounds of the plant extract.
Highlights
Populations of all the continents use plants for their various needs in terms of shelter, clothing, food, flavours and fragrances as not the least, medicines [1]
The anti-inflammatory activity of W. amplissima HEA was assessed in vivo through its anti-edematous and analgesic effects as summarized in table 1 and table 2
The present study demonstrated that hydro acetonic extract (HAE) of W. amplissima possess potential pharmacological properties including antioxidant, anti-edematous, analgesic and inhibiting enzymes effects
Summary
Populations of all the continents use plants for their various needs in terms of shelter, clothing, food, flavours and fragrances as not the least, medicines [1]. The last decade has witnessed a growing number of populations who rely on traditional remedies derived from medicinal plant and tremendous progress in medicinal plant research. This worldwide trend towards the use of natural plant remedies has created an enormous need for information about the properties and uses of the medicinal plant for the treatment of minor ailments to more complex diseases. In the central region of Burkina Faso, species belonging to this family are well known and used by traditional practitioners to treat several diseases. Ethnopharmacological studies report the use of this species in folk medicine to treat vomiting, severe diarrheas, mucosa, skin and bronchus inflammation; inflammatory diseases, fever and allergies. The roots of the plant are used to treat anemia, hemorrhages and as a fortifying [4]
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
