Abstract
Aim: The aim of the study was to employ Nigella sativa oil in the synthesis of silver nanoparticles and to assess the anti-inflammatory activity of the Nigella sativa oil mediated silver nanoparticles. Background: The introduction of Nanoparticles (NPs) has transFigured many fields like medicine, nutrition and electronics. The usage of nanotechnology in medicine particularly for drug delivery is revealed to have numerous benefits. Nanoparticles are being used to decrease toxicity and side effects that drugs may impose to the patient. Nigella sativa is considered as a miracle seed. It has been reported to have anti-inflammatory, diuretic, and antihypertensive activity. Plant mediated biological synthesis of nanoparticles is simple and eco-friendly method. This work therefore was aimed to synthesize Nigella sativa oil mediated silver nanoparticles and evaluate its anti-inflammatory activity. Materials and Methods: Nigella sativa oil mediated silver nanoparticles were synthesised by short term (1 day) interaction of Nigella sativa seed extract (1 ml) with 2mM AgNO3 solution and centrifuged to obtain silver nanoparticles. The nanoparticles were characterised by UV-Visible spectrophotometer, FTIR and Scanning Electron Microscopy (SEM). Further the oil mediated AgNPs were evaluated for anti-inflammatory activity by in vitro and in vivo methods. Results: Nigella sativa oil mediated AgNPs were biofabricated with ease and exhibited good anti-inflammatory activity compared to standard. Conclusion: This study concludes that Nigella sativa seed oil mediated silver nanoparticles have the potential to be used as an effective antioxidant. Hence, it may be used in many medicinal applications to treat inflammation.
Highlights
Noble metals, such as silver and gold, due to their specific and unique chemical, biological, and physical properties have attained tremendous interest in nanomedicine.[1]
Nigella sativa oil mediated AgNPs were biofabricated with ease and exhibited good anti-inflammatory activity compared to standard
The present study revealed concentration dependent inhibition of protein (Bovine serum albumin) denaturation by ethanolic seed extract of Nigella sativa and AgNPs
Summary
Noble metals, such as silver and gold, due to their specific and unique chemical, biological, and physical properties have attained tremendous interest in nanomedicine.[1] The phytochemical synthesis of silver nanoparticles (SNPs) by plants or seed extracts has unique role in the field of nanotechnology and nanomedicine as it provides alternative therapeutic options. Prostaglandins (PGs) are effective proinflammatory mediators attained from arachidonic acid metabolism by cyclooxygenases and play an important role in modulating a number of pathophysiological conditions like inflammation and allergic immune responses.[3]. The role of herbal medications to boost common health conditions is always considered effective.[4] Herbal therapies are often used to treat inflammation, their molecular and cellular bases of action are less elucidated. Thymoquinone (2-isopropyl-5-methyl-1,4-benzoquinone) is the most copious phytoconstituent of the seed extract and possess antioxidant and anti-inflammatory activities.[6]
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