Antihyperlipidemic and hepatoprotective properties of alkali- and enzyme-extractable polysaccharides by Dictyophora indusiata

Wenshuai Wang,Jianjun Zhang,Zhen Song,Xinling Song,Zheng Gao,Le Jia,Yanbo Feng,Fangfang Yuan,Honghong Liu,Yiwen Zhang

doi:10.1038/s41598-019-50717-9

Abstract

Hyperlipidemia, a very common disease throughout the world, usually gives rise to severe liver damages. The current experiment was to investigate the antihyperlipidemic and hepatoprotective properties of alkali- and enzyme-extractable Dictyophora indusiata polysaccharides (Al-DPS and En-DPS) on the hyperlipidemic mice. The results of animal experiment in vivo showed that treatment with Al-DPS or En-DPS could improve the excessive level of lipid profiles in serum and liver, as well as strengthen antioxidant status. In addition, the histopathological observations of liver testified that polysaccharides were capable of attenuating hepatic cell injury. The primary structural features of Al-DPS and En-DPS were demonstrated by HPGPC, HPLC, FT-IR and NMR. Glucose tolerance test manifested that polysaccharides were able to restrain the rise of blood sugar. The results indicated that Al-DPS and En-DPS may be considered as novel compounds to treat hyperlipidemia and also act as hepatoprotective agents.

Highlights

Hyperlipidemia is a systemic disease, which is characterized by elevated lipid levels in blood including total cholesterol (TC), total glyceride (TG), and low-density lipoprotein cholesterol (LDL-C) and so on[1]
The mice treated with Al-DPS or En-DPS did not exhibit any gross behavioral changes, toxic responses or deaths even at a dose of 1200 mg/kg during the feeding period in comparison with control group
All the polysaccharide groups and positive control group (PC) group showed lower weight compared with model control group (MC) group, indicating Al-DPS, En-DPS and simvastatin had potential contributions in reducing the gain of weight

Summary

Introduction

Hyperlipidemia is a systemic disease, which is characterized by elevated lipid levels in blood including total cholesterol (TC), total glyceride (TG), and low-density lipoprotein cholesterol (LDL-C) and so on[1]. It is an important risk factor leading to fatty liver, cardiovascular disease and atherosclerosis[2], and becomes the first killer of human health. Dictyophora indusiata, a kind of famous edible mushroom, is widely distributed and eaten around the world It is designated as the queen of the mushrooms rich in various nutrients including polysaccharide, protein, mineral, vitamin, amino acids, riboflavin and nicotinic acid[14,15]. The structures of alkali- and enzyme-extractable Dictyophora indusiata polysaccharides (Al-DPS and En-DPS) were preliminarily explored, and the animal experiment was performed to investigate the hypolipidemic and hepatoprotective effect of polysaccharides subsequently

Methods

Results

Conclusion