Antihyperglycemic activity of nanoemulsion of brown algae (Sargassum sp.). Ethanol extract in glucose tolerance test in male mice

Abstract

Nanoemulsion technology has been widely developed and applied to extracts of natural materials to enhance bioavailability and medicinal effects. This study aimed to determine the effectiveness of the Sargassum sp. ethanol extract nanoemulsions as an antihyperglycemic agent against fasting blood glucose levels in mice. The nanoemulsion formulation used Sargassum sp. extract and some additional ingredients, including chitosan, sodium tripolyphosphate, and tween 80. The antihyperglycemic test consisted of four groups, which were randomly selected. Treatment group (I) was given a nanoemulsion base without algae extract with a volume of 0.2mL/20gramBW; treatment group (II) was given glibenclamide at a dose of 0.52mg/20gramBW in 0.5% carboxymethylcellulose sodium (NaCMC) suspension with a volume of 0.2mL/20gramBW; treatment group (III) was given Sargassum sp. ethanol extract at a dose of 0.66mg/20 gramBW in 0.5% Na CMC suspension with a volume of 0.2mL/20gramBW; the treatment group (IV) was given formula of nanoemulsions of ethanol extract Sargassum sp with a volume of 0.2mL/20gramBW equivalent to a dose concentration Sargassum sp. ethanol extract of 0.66mg/20gramBW. The size of the nanoemulsion particles of the Sargassum sp. extract was 341.5-296.5nm with a zeta potential of 19.4-16.9mv. Treatment group (II) had the same antihyperglycemic effect as treatment group (IV). In contrast, treatment groups (I) and (III) had a relatively lower antihyperglycemic effect. This suggests that the Sargassum sp. extract nanoemulsion formula can be used as an alternative antihyperglycemic agent.