Abstract
Hypercholesterolemia is a condition characterized by increased levels of total cholesterol in the blood. Pheophytin is the result of degradation of chlorophyll which is the dominant pigment in green tea, and has been shown to have the same antioxidant properties as - carotene. This study aims to determine the antihypercholesterolemia effect and effective dose of pheophytin isolates and acute toxicity tests to determine the safety level of pheophytin isolates.
 Antihipocolesterolemia test uses induction of high-fat feed that is a mixture of standard food, pork oil and quail egg yolk (16: 3: 1). While the acute toxicity test is carried out based on OECD 423, Clinical symptoms that arise for 3 hours are observed intensively. Histopathological examination data of liver and kidney organs were performed on day 14. Data obtained in the form of LD 50 and clinical symptoms that arise as well as histopathological results were analyzed qualitatively.
 Statistical test results of percent reduction in total cholesterol levels showed a significant difference between the negative control group and the pheophytin isolates of the three doses. This proves that pheophytin isolates can have antihypercholesterolemic effect with an effective dose of 10 mg/kgBW. Histopathological examination results of aorta and liver of rats showed that the higher the dose of pheophytin isolates, the lighter the degree of fatty acid. While the toxicity test results showed that the LD50 value of pheophytin isolates was more than 2000 mg / kgBW (non-toxic category). However, pheophytin isolate with LD50 is more than 2000 mg/kgBW which has shown the presence of pancreatic degeneration and hydropic degeneration, but necrosis has not occurred yet.
Highlights
Cholesterol is a yellowish fat, like wax produced by the body, especially in the liver [1].Cholesterol is one component of fat that is needed by the body in addition to carbohydrates, proteins, vitamins and minerals
An acute toxicity test needs to be done in order to determine the safety of pheophytin isolates so that they can be applied to the community as antihypercholesterolemia
If there are mice that die, the dosage of pheophytin isolates is reduced according to the dose rating determined by Organization for Economic Cooperation and Development method (OECD) 423
Summary
Cholesterol is a yellowish fat, like wax produced by the body, especially in the liver [1].Cholesterol is one component of fat that is needed by the body in addition to carbohydrates, proteins, vitamins and minerals. Research needs to be done to see the antihypercholesterolemia effect of pheophytin isolates in green tea. An acute toxicity test needs to be done in order to determine the safety of pheophytin isolates so that they can be applied to the community as antihypercholesterolemia. Group IV, V and VI : HFD days to 0-60, and suspension of pheophytin isolates 10; 20; 40 mg/kgBW days to 61-67 by oral
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
