Antihemorrhagic and Antimicrobial Property of Cotton Fabric Treated with Medicinal Herb Alfalfa

Abstract

Abstract In this work, medicinal plant alfalfa leaf extract was used as an antihemorrhagic, antimicrobial, and antifungal agent and applied to cotton fabrics. The medicinal herb alfalfa leaf was extracted using the ethanol and methanol solvents in the Soxhlet apparatus. The 50-µL, 100-µL, and 150-µL concentrations of extracted solution were tested against the positive and negative bacteria, namely Staphylococcus, Escherichia coli, Pseudomonas aeruginosa, Bacillus subtilis, and the yeast fungi. The zone of inhibition was measured in each concentration. The 150-µL extract concentration in methanol extract showed good antibacterial activity against Staphylococcus and P. aeruginosa bacteria compared with the ethanol extract. The treated cotton fabrics were assessed for their antimicrobial property, and the zone of inhibition was found to be in the range of 13–15 mm. Then the antihemorrhagic property was assessed in both ethanol extract and methanol extract solution alone. The ethanol extracts showed a minimum time for clotting the blood, i.e., 2.2 min. The treated fabrics were assessed for the time taken to clot blood, and it was found that it took 1.2–1.4 min, which was improved when compared with the normal blood clotting time of 2–2.3 min. Biocompatibility Chorioallantoic membrane (CAM) preparation helps us to know the endpoints like hemorrhage, vascularlysis, coagulation, and irritation score of treated and untreated cotton fabric. The test is similar to the effects of the rabbit skin testing treatment, which shows the result of zero value for endpoints. The Medicago sativa herb exhibited good antimicrobial and antihemorrhagic properties for medicinal applications.