ANTIHEMOPHILIC FACTOR IN CROSS-CIRCULATED NORMAL AND HEMOPHILIC DOGS.

Abstract

The technique of cross circulation was used to study antihemophilic factor ( AHF) in normal and hemophilic dogs. Following cross circulation of 60 min, the plasma AHF of the hemophilic animals had increased to about 50% of the normal level. This increase was not accompanied by a corresponding decrease in the AHF in the plasma of the normal dog. In other experiments, splenectomized normal dogs were cross circulated with hemophilic dogs. Without a spleen, the normal dog was unable to maintain its plasma AHF during the shunting procedure. These studies indicate that the spleen is important in the homeostasis of antihemophilic factor.