Abstract
Using immunofluorescence methods, 3 antisera respectively stain 3 groups of mucous cells of the human gastrointestinal tract, showing specific antigens for each group of cells. The antigens of the first group, the M1 antigens, were principally associated with columnar cells of the gastric epithelium, the M2 antigens with mucous cells of gastric and Brünner's glands, and the M3 antigen with the goblet cells of the intestinal mucosa. The gastric M antigens normally detectable in stomach and duodenum (but not in colon) were expressed in certain colonic tumours (benign or malignant) and in adjacent mucosa. They are always present with the intestinal M3 antigen. In 100 colonic adenocarcinomas, the intestinal M3 antigen was found in 53 cases, gastric M1 antigens in 29 cases, and gastric M2 antigens in 10 cases, always with the two other M antigens. A good correlation could be established between the association of M antigens and the histological type of tumour.
Highlights
The antigens of the first group, the MI antigens, were principally associated with columnar cells of the gastric epithelium, the M2 antigens with mucous cells of gastric and Brunner's glands, and the M3 antigen with the goblet cells of the intestinal mucosa
IN A RECENT WORK (Bara et al, 1978) we described an antigen (SGA, termed M3) that was associated with goblet cells in normal intestinal mucosa but not in normal stomach, and which appeared as an aberrant antigen in certain gastric tumours
These results prompted us to investigate whether gastric antigens associated with mucous cells not present in the normal colonic mucosa could be seen in colonic tumours
Summary
Gastrointestinal tissue samples were taken from different parts of the gastrointestinal tract not more than 1 h after surgery. One hundred samples of colonic mucosa were taken near adenocarcinomas of the bowel: 3 of them > 10 cm and the others > 2 cm away from the tumour. HMW proteins from pure endocervical-type ovarian mucinous cyst gave by immunoprecipitation 2 main lines with ovarian and gastric antigens. This antiserum stained by immunofluorescence only the surface epithelium of gastric mucosa. On the other hand, when we absorbed ani antiserum against gastric HMW proteins w%vith the pure endocervical-type ovarian mucinous cyst fluid, this antiserum did not precipitate with gastric or ovarian antigens, but stained the gastric glands by immunofluoreseence. Wt (HMW) proteins.-The ovarian, gastric and colonic lyophilized materials were fractionated individually according to the method described by Andre and Descos (1975). Supernatants were dialysed against deionized water and against Tris HCI buffer (O1M pH 8) containing 2M NaCl, and successively chromatographed on Sepharose 6B and Sepharose 2B (Pharmacia, Uppsala, Sweden) in the same buffer
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