Antigenotoxicity, Cytogenetic Assay measuring Chromosomal Aberration and Antimicrobial Activity of Methanolic Extract of Microalgae: Nannochloropsis oculate

Abstract

The aim of the present study was to investigate the anti-genotoxic effect of methanolic extract of microalgae-Nannochloropsis oculata and also to determine the antibacterial from marine microalga extract in methanol. Genotoxicity studies were conducted for screening of mutagenicity using Ames test, direct plate incorporation method using tester strain of Salmonella typhimurium viz., TA 98 and hence to prove this as an antigenotoxicant. The mutagenicity assay was performed with 3 dose levels (0.312, 1.25 and 5.0mg/ml) using all two tester strains without metabolic activation system (S9 fraction). Inhibition in the background lawn or reduction in revertant colonies was observed at tested dose concentration in two tester strains without metabolic activation system. Further, more works are to be carried out to find the exact mechanism of its Geno protective nature. The methanolic extract of Nannochloropsis oculata was also examined for antimicrobial activity by well-diffusion method. The extract showed high inhibition against the growth of Bacillus Subtilis when compared to the other tested strains. All microorganisms tested are susceptible to the treatment with methanolic extract of Nannochloropsis oculata in a concentration dependent manner.