Antigenotoxic Effect of Piperine in Broiler Chickens Intoxicated with Aflatoxin B1.

Verônica Da Silva Cardoso,Maria Das Graças Miranda Danelli,Glória Maria Direito,Marco Edilson Freire De Lima,Cristina Amorim Ribeiro De Lima,Lúcia Helena Pinto Da Silva,Alane Beatriz Vermelho,Jéssica Mendes De Oliveira

doi:10.3390/toxins8110316

Abstract

Piperine is an abundant amide extracted from black pepper seeds which has been shown to have protective effects against cytotoxic and genotoxic carcinogenesis induced by certain chemical carcinogens and aflatoxin B1 (AFB1) in vitro. The aim of this work was to study, in vivo, the antigenotoxic potential of feed-added piperine on broiler chickens experimentally intoxicated with AFB1, using micronucleus and comet assays. The antigenotoxicity assessment of 9-day-old chicks was performed on a total of 60 chickens divided into four groups of 15 broilers each: (C) control, (P) 60 mg·piperine kg−1 feed, (A) 0.5 mg·AFB1·kg−1 body weight, (daily by oral route), and (P + A) co-treatment with piperine and AFB1. The experiment was conducted for 26 days. Chicks intoxicated with AFB1 showed significant genotoxic effects in the first 24 h post intoxication, and the effects remained in the other periods analyzed (48, 72, and 96 h and 26 days of treatment). The DNA damage in peripheral blood cells, the number of erythrocytes with micronuclei, and polychromatic-to-normochromatic erythrocyte ratio were significantly reduced or absent in the piperine/AFB1 group. No significant differences were observed between the group piperine/AFB1 and the control and piperine-alone groups. The addition 60 mg·kg−1 of piperine to the diet of the broiler chicks was safe, promoting beneficial effects in poultry health with respect to the toxic effects 0.5 mg·AFB1·kg−1 body weight.

Highlights

IntroductionAflatoxicosis is a disease resulting from the ingestion of aflatoxin in contaminated food and feed, and has become one of the most widespread and important diseases in the poultry industry [1,2,3]
Aflatoxicosis is a disease resulting from the ingestion of aflatoxin in contaminated food and feed, and has become one of the most widespread and important diseases in the poultry industry [1,2,3].the increased human consumption of chicken has placed new demands on the poultry industry which require adaptation
The supplementation of piperine in aflatoxin contaminated broiler diets maintained production rates in early stage, growth and total period significantly when compared to aflatoxin without piperine

Summary

Introduction

Aflatoxicosis is a disease resulting from the ingestion of aflatoxin in contaminated food and feed, and has become one of the most widespread and important diseases in the poultry industry [1,2,3]. The increased human consumption of chicken has placed new demands on the poultry industry which require adaptation. Most consumer products are required to be safe and free of pathogens or substances that could be harmful to human health [4,5,6,7]. AFB1 is the subtype most commonly found in contaminated foods, and is considered the most toxic of the subtypes, followed by aflatoxin G1 , G2 , and B2 [9,10]. Aflatoxins are chemically stable and it is difficult to inactivate them through physical or chemical methods [9]

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