Abstract
Whey protein concentrate (WPC) hydrolysates were prepared with trypsin and treated with microfiltration (MF) and/or ultrafiltration (UF) membrane. HPLC and the immunoreactivity test in vitro (inhibition enzyme-linked immunosorbent assay: inhibition ELISA) were performed on these hydrolysates. Although the insoluble materials were removed from the hydrolysates, no differences were recognized in the patterns of HPLC before or after MF treatment. The main peaks of fraction obtained by MF membrane treatment showed a molecular weight range of 1000 to 6000. On the other hand, fractions by UF membrane treatment were between 1000 and 3000. From the results of the inhibition ELISA, it was recognized that the antigenicity of fractions after UF membrane treatment dropped fron 1/30 to 1/200 compared with the WPC hydrolysate.
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