Antigenicity of two established tumorogenic cell lines estimated by the migration inhibition test.

Abstract

Summarya. MIT used in the present work was found to be a useful method for the evaluation of sensitization occurring in oncological experimental systems, where transplantation tests may fail due to high virulence of the tumor.b. Both cell lines used, L-1 and L-2c, which are established tumorogenic cell lines derived from the C3H strain, sensitize isogenic hosts when injected sc.c. No cross-reaction between the two cell lines could be demonstrated by the MIT as used in this work.d. Both cell lines sensitize hosts of DBA strain across H-2. However, only slight signs of cross-reaction were seen, when DBA spleen sensitized this way was challenged by C3H spleen cell homogenate.e. Experiments on tumors derived from the tumorogenic cell lines show cross-reactions with the original cell lines.f. Slight signs of autoimmunization were observed in C3H mice after sensitization by the transformed cell lines, when challenged by the normal C3H spleen homogenate.