Abstract
AbstractSeveral poly‐L‐proline batches, as well as their dinitrophenyl derivatives, were synthesized and characterized. Their immunogenicity in rabbits, guinea pigs and mice was investigated, and the specificity of the antibodies formed was determined. For sensitive detection of anti‐polyprolyl antibodies, polyprolyl bacteriophage T4 was prepared. Polyproline was in this case attached to the bacteriophage via 1,3‐difluoro‐4,6‐dinitrobenzene.Dinitrophenyl poly‐L‐proline is immunogenic in rabbit and guinea pig, but essentially nonimmunogenic in the mouse. No genetic differences in the antibody response were found either in strains 2 and 13 of guinea pigs, where both strains were good antibody producers, or in several strains of mice, all of which were essentially unresponsive. Poly‐L‐proline, considered nonimmunogenic in rabbits by previous criteria, led to a low but significant antibody response, when tested with polyprolyl bacteriophage.
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