Abstract
Precipitating, complement-fixing and neutralizing antibody was produced in rabbits by immunization with a partially purified human pituitary FSH preparation. Apparent serological specificity of this antiserum was increased after absorption with human serum and HCG. Attempts were made to examine immunologic carbohydrate determinant groups of human pituitary FSH by complement-fixation inhibition reactions between the absorbed antiserum and homogeneous human pituitary FSH preparations, using mono- and disaccharides, and by treatment of the hormone preparations with several carbohydrate-hydrolyzing enzymes. Of the saccharides tested, galactose, β-methyl- D-galactopyranoside lactose and melibiose were active in the inhibition test, and the disaccharides were more effective than the monosaccharides on an equimolar basis. Ovarian weight augmentation activity of FSH was completely abolished, but its complement-fixing property was not affected after treatment with neuraminidase. Both biologic and immunologic prope...
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