Abstract
Abstract Suspensions of washed leukocytes from immunized rabbits release histamine on contact with the immunizing antigen. This allergic response is calcium- and temperature-dependent and occurs in the absence of added plasma or serum. The leukocytes become reactive within 5 days after a single injection of antigen. The responsiveness of the cells reaches a peak in 2 weeks and persists for at least 8 weeks. Cells from hyperimmunized rabbits do not release histamine in the presence of antigen. Histamine release from rabbit leukocytes is not accompanied by enhanced efflux of 86Rubidium or the uptake of trypan blue. Studies with DNP conjugates suggest that a multiplicity of determinants, all possessing the DNP group, participate in histamine release from rabbit leukocytes. These cells lose their ability to release histamine after incubation with antigen in the absence of calcium. Although leukocytic histamine release may be observed when rabbit PCA tests are negative, there is good correlation between the two reactions.
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