Abstract
Friend leukemia virus (FLV) erythroleukemic cells cultured in medium containing FLV-immune serum from dormant FLV-infected mice undergo modulation of FLV cell surface antigens. Modulation was determined by an increased resistance to FLV antibody-mediated complement-dependent lysis and was associated temporally with the capping of FLV-immune complexes at the cell surface. Modulated cells regained their susceptibility to FLV antibody-mediated complement-dependent lysis when transferred to medium containing normal mouse serum. After 48 h of culture in FLV-immune serum, 26% of the FLV erythroleukemic cells were devoid of FLV cell surface antigens as demonstrated by immunofluoresence. Antigenic modulation occurred to a greater extent in cells maintained in logarithmic growth than in cells in GO or resting phase. FLV-antigenic modulation is discussed as a possible mechanism by which antibody induces and maintains FLV-transformed cells in a dormant state.
Highlights
FLC-745 cells were cultured in medium containing FLV-immune serum (FVIS) to determine the effect of antibodies directed against Friend leukemia virus (FLV) cell surface antigens on cell growth, viability, and susceptibility to FVIS-mediated C-dependent cytolysis
One subculture was diluted with fresh medium containing FVIS or Normal mouse serum (NMS) to maintain the cells in logarithmic growth phase
Friend leukemia virus (FLV) erythroleukemic cells cultured in medium containing FLV-immune serum from dormant FLV-infected mice undergo modulation of FLV cell surface antigens
Summary
Clone 745 of Friend erythroleukemia cells (FLC-745) were obtained from the Genetic Mutant Cell Repository, Institute for Medical Research, Camden, N. J., which has designated this clone GM86. The cell line was derived from solid leukemic tumors passaged in DBA/2 mice [37]. The cells were maintained in suspension culture in 75 cm plastic tissue culture flasks, and transferred twice weekly at a final concentration of 10~ cells/ml of culture medium, consisting of RPMI 1640 (pH 7.0) with 20% heat-inactivated fetal bovine serum and 100 tLg/ml streptomycin, 100 U/ml penicillin, 100 t~g/ml gentamycin, and 2 mM glutamine. All cultures were incubated at 37°C in a humidified chamber containing a 5% CO2 atmosphere
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