Abstract
CEA samples were prepared individually from liver metastases of gastric and colonic cancer, and purified with gel filtrations, block electrophoresis and Con A affinity chromatography (the latter was not performed in all cases). Anti‐CEA antisera were produced in 7 and 4 guinea pigs against CEA of gastric cancer and colonic cancer respectively. Upon extensive absorptions with PCA extracts of normal tissues (rich of NCA) and NCA‐2, their anti‐CEA activities were examined on micro‐Ouchterlony plates and by immunofluorescence techniques.There were two types of anti‐CEA antibodies, as observed in our previous study [9], in which crude samples of CEA were utilized as immunogens: a type that showed identical reaction to CEA of adenocarcinomas of the digestive system, squamous cell carcinomas of endodermally derived organs, medullary carcinoma of the thyroid as well as tissues of fetal gastro‐intestinal tract but not to others, while the other type was proven to be specific to gastric or colonic cancer depending on cancer tissues from which the immunized CEA was extracted. In immunofluorescence study, no reaction of these organ‐specific anti‐CEA antibodies to fetal tissues was observed.Further, CEA‐M (Con A binding fraction) and CEA‐P (Con A non‐binding fraction) separated by Con A affinity chromatography, showed identical reaction to any types of anti‐CEA antibodies, though CEA‐P was apparently less antigenic.
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