Antigenic characterization of foot-and-mouth disease virus serotype Asia1 field isolates using polyclonal and monoclonal antibodies

Abstract

Foot-and-mouth disease virus (FMDV) serotype Asia1 field isolates ( n=100) were compared using a panel of 11 monoclonal antibodies (Mab) in sandwich ELISA. The majority (over 89%) of the isolates showed either homologous (76% and above reactivity) or reduced affinity (20–75% reactivity) for the Mabs 2A, 13, 40, 34 and 81, suggesting that these Mab binding epitopes are conserved, whereas a more variable reactivity was observed for the Mabs B3, 1A, 24, 72, 82 and 89. Polyclonal relationship (‘ r’ value) of the field isolates in liquid phase blocking (LPB) ELISA was examined, and the mean ‘ r’ value was 0.62 relative to vaccine virus IND 63/72. Some of the field isolates ( n=34) were tested in virus neutralization test (VNT) and showed an ‘ r’ value of >0.40. Although a minor antigenic difference was observed in the Mab profiling study, there has not been large antigenic divergence between reference virus and field viruses, thereby providing evidence of wide antigenic coverage of the vaccine strain.