Abstract
BackgroundAvian reovirus (ARV) causes arthritis, tenosynovitis, runting-stunting syndrome (RSS), malabsorption syndrome (MAS) and immunosuppression in chickens. σB is one of the major structural proteins of ARV, which is able to induce group-specific antibodies against the virus.Methods and ResultsThe present study described the identification of two linear B-cell epitopes in ARV σB through expressing a set of partially overlapping and consecutive truncated peptides spanning σB screened with two monoclonal antibodies (mAbs) 1F4 and 1H3-1.The data indicated that 21KTPACW26 (epitope A) and 32WDTVTFH38 (epitope B) were minimal determinants of the linear B cell epitopes. Antibodies present in the serum of ARV-positive chickens recognized the minimal linear epitopes in Western blot analyses. By sequence alignment analysis, we determined that the epitopes A and B were not conserved among ARV, duck reovirus (DRV) and turkey reovirus (TRV) strains. Western blot assays, confirmed that epitopes A and B were ARV-specific epitopes, and they could not react with the corresponding peptides of DRV and TRV.Conclusions and SignificanceWe identified 21KTPACW26 and 32WDTVTFH38 as σB -specific epitopes recognized by mAbs 1F4 and 1H3-1, respectively. The results in this study may have potential applications in development of diagnostic techniques and epitope-based marker vaccines against ARV groups.
Highlights
Avian reovirus (ARV) belongs to the genus Orthoreovirus in the family Reoviridae
The results showed that the monoclonal antibodies (mAbs) 1F4 and 1H3 couldn’t neutralize the virus
The results indicate that the mAb (1H3-1) could react with both the Pep (31-45 aa) and Pep (32-46 aa), which indicated that the epitope of mAb (1H3-1) might be located in peptide (32WDTVTFHVPDVIRV45) (Figure 2)
Summary
Avian reovirus (ARV) belongs to the genus Orthoreovirus in the family Reoviridae. It was first isolated from chickens in 1954 [1]. The S1133 virus is the standard challenge virus for VA and is in most VA vaccines around the world It was isolated from sick chickens around 50 years ago [12]. Avian reovirus (ARV) causes arthritis, tenosynovitis, runting-stunting syndrome (RSS), malabsorption syndrome (MAS) and immunosuppression in chickens. Methods and Results: The present study described the identification of two linear B-cell epitopes in ARV σB through expressing a set of partially overlapping and consecutive truncated peptides spanning σB screened with two monoclonal antibodies (mAbs) 1F4 and 1H3-1.The data indicated that 21KTPACW26 (epitope A) and 32WDTVTFH38 (epitope B) were minimal determinants of the linear B cell epitopes. Antibodies present in the serum of ARV-positive chickens recognized the minimal linear epitopes in Western blot analyses. The results in this study may have potential applications in development of diagnostic techniques and epitope-based marker vaccines against ARV groups
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