Abstract
The cellular basis of the genetic control of the immune response to poly(LTyr, LGlu)-polyDLAla--polyLLys [(T,G)-A--L] in SJL (H-2s, low responder) mice has been investigated using T-cell factors. Thymocytes of SJL origin were educated to (T,G)-A--L and tested for their ability to produce an antigen-specific factor capable of cooperating in vivo with bone marrow cells of either SJL or C3H.SW (high responder) origin. SJL T cells were found to be incapable of producing such a cooperative factor, in contrast with results previously obtained with C3H/HeJ (low responders) and C3H.SW strains. Moreover, SJL bone marrow cells did not produce an antibody response to (T,G)-A--L, even when combined with factor produced by high responder (C3H.SW) mice. Thus, both T and B cells appear to be defective in the SJL strain in the response to (T,G)-A--L.
Highlights
The factors were tested for their ability to cooperate with B cells of high responder origin, by transfer together with C3H.SW B cells and (T,G)-A--L into irradiated C3H.SW recipients
Ability of T-Cell Factors Produced by C3H.SW and SJL T Cells to Cooperate with C3H.SW B Cells
We have previously suggested that the cellular defect in C3H/HeJ (H-2k ) low responders lies in the ability of the (T, G)-A--L-specific B cells to receive and respond to a T-cell signal imparted by the soluble T-cell factor [1]
Summary
The following multichain synthetic polypeptides were used : (a) Poly (LTyr,LGlu)-Poly DLAla--polyLLys, abbreviated as (T,G)-A--L, batch no. (b) Poly(LTyr,LGlu)-polyLPro-polyLLys, abbreviated as (T,G)-Pro--L, batch no . The synthesis and characterization of such immunogens have been previously described [5, 6]. Mice of the strains C3H .SW (H-2°) and SJL/J (H-28), bred at The Experimental Animal Unit, The Weizmann Institute of Science, Rehovot, Israel, were used. The methods used for the preparation of T-cell factors t Recipient of a short-term European Molecular Biology Organization fellowship
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