Abstract
Rat hepatoma cell line McA Rh 7777 produces α-fetoprotein (AFP)in vitro. This line is subcloned into ATP-producing and nonproducing subclones. The AFP-negative clone is used to generate hybridomas producing monoclonal antibodies recognizing AFT− and AFP+ clones. One of these hybridomas (A2/3) reacts only with AFP− clones. Double staining for AFP and A2/3 antigen (AG A2/3) reveals alternative expression of AFP and AG A2/3 on McA Rh 7777 hepatoma and its clones. The antigen is present on transplantable H-27 hepatoma (AFP−) and is not expressed by Zaidel hepatoma cells (AFP+). AG A2/3 is absent from the liver of normal adult rats and rat embryos. The antigen is inducedin vivo by the injection of lead nitrate andin vitro by treatment of McA RH 7777 hepatoma and its clones with cadmium chloride. It is likely that AG A2/3 is a cell stress protein.
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