Abstract
Surface modified poly-ɛ-caprolactone microspheres as an antigen carrier was explored in a fish model. Outer membrane vesicles of Edwardsiella tarda adsorbed on to surface modified poly-ɛ-caprolactone microspheres with chitosan and alginate induces both innate and adaptive immune responses which persist up to 63 days of post immunization through parenteral immunization unlike that of free and FIA adjuvented antigens. These results highlight the role of these microspheres as an adjuvant/antigen carrier in fish.
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