Abstract
Silver particles were prepared by dewetting Ag films coated on glass using a fiber laser. The size of the particles was controlled in the range of 92 nm–1.2 μm by adjusting the thickness of the Ag film. The structural properties and surface roughness of the particles were evaluated by means of scanning electron microscopy. In addition, the antifungal activity of the Ag particles was examined using spore suspensions of Colletotrichum gloeosporioides. It is shown that particles with a size of 1.2 μm achieved 100% inhibition of conidia growth of C. gloeosporioides after a contact time of just 5 min. Furthermore, the smaller particles also achieved good antifungal activity given a longer contact time. Similar results were observed for spore germination and pathogenicity tests performed on mango fruit and leaves. Overall, the results confirm that Ag particles have an excellent antifungal effect on C. gloeosporioides.
Highlights
Mango (Mangifera indica L.) is a popular fruit with a strong and distinctive flavor that is widely grown in many tropical and subtropical countries [1]
The effect of the Ag particles on the melanin synthesis behavior of the C. gloeosporioides spores was evaluated by performing the RT-PCT analysis of five related genes, namely polyketide synthase (PKS), tetra-HN reductase scytalone (THR), scytalone dehydratase (SCD), exo-glucocanase (Ecg) and pectate lyase (PEL)
It was seen that the particle size increased with an increasing Ag film thickness and increased laser repetition rate
Summary
Mango (Mangifera indica L.) is a popular fruit with a strong and distinctive flavor that is widely grown in many tropical and subtropical countries [1]. Various techniques are available for the production of micro- and nanoscale metallic particles, including mechanochemical methods [16], green synthesis [17], spray pyrolysis [18], sol-gel [19], chemical vapor deposition [20] and dewetting [21]. In the latter method, a metallic film is deposited on a glass substrate annealed, resulting in a dewetting phenomenon that minimizes the total energy of the free surfaces of the film and substrate. The antifungal activity of the Ag particles is investigated by examining the conidia growth and germination rate of C. gloeosporioides spores in both in vitro experiments and in vivo experiments performed using mango fruit and leaves
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