Abstract
Water extracts at the concentration of 0.5, 1, 2, 3 and 4%, of propolis were prepared and investigated for inhibition of Alternaria alternata, Aspergillus niger, Aspergillus parasiticus, Botrytis cinerea, Fusarium oxysporum i.sp. melonis and Penicillium digitatum in culture media. The concentration of 4% was the most inhibitory of 5 concentrations studied. Microorganisms the most effected from all the concentrations among tested fungi were Alternaria alternata and Penicillium digitatum . The concentration of 4% of propolis extract showed more than 50% inhibition against all tested microorganisms.
Highlights
Propolis is a resinous hive product collected from plant buds by bees
Propolis extract at concentrations of 0.5,1,2 and 3% less effected on mycelial growth of test microorganisms but 4%
While all the concentrations showed inhibitory effect about 45-60% against A. altemata until six days of incubation, inhibitory activity was partly decreased on ten days of incubation
Summary
Propolis is a resinous hive product collected from plant buds by bees. Bee products such as honey, pollen and propolis are used for the treatment of several diseases. Investigations have indicated that propolis contains wax, flavonoids, amino acids, essential oils, pollen, minerals and organic matters (Walker and Crane 1987, Crane 1990, Scheller etaL, 1990). Water extracts of propolis have been used for the treatment of several diseases observed in mammals (Lindertelser 1967, Ghisalberti 1979, Konig 1986, Walker and Crane 1987, Orgen 1988, Rosential 1989, Scheller etal., 1989, Crane 1990, Grange and Davey 1990, Tatefuji et al, 1993, Itah et al, 1994, Digrak et al, 1995, Marcucci 1995).
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