Abstract
A laboratory experiment was conducted to study the efficacy of some botanicals against seed-borne fungi isolated from barley. Alternaria alternata was the most frequently isolated fungi followed by Rhizopus spp., and Mucor spp., determined by plating the seeds on both standard blotter and agar plate method. Leaf extracts of five plants viz., Eucalyptus globulus, Calotropis procera, Melia azedarach, Datura stramonium and Acalypha indica @ 5%, 10% and 20% concentration were evaluated against A. alternata. The results revealed that all the plant extracts significantly inhibited the mycelial growth of A. alternata. Effect of these five plant extracts varied with the concentrations. Leaf extract of E. globulus at 20% concentration caused highest inhibition of mycelial growth of A. alternata (52.6%) followed by C. procera (50.88%), M. azedarach (48.21%) and D. stramonium (47.42%), whereas the lowest inhibition (37.52) of mycelial growth was recorded at 5% leaf extract concentration in case of A. indica as compared to control. However, seed treatment at 20% concentration of all the tested plant extracts was also found to be effective in eliminating majority of fungi and reducing the relative frequency of seed-borne fungi occurring on the seeds and also results in percent germination increase in both standard blotter and agar plate method over control.
Highlights
Barley is an important rabi season crop of India and plays a major role in barley production all over the world
Leaf extract of E. globulus at 20% concentration caused highest inhibition of mycelial growth of A. alternata (52.6%) followed by C. procera (50.88%), M. azedarach (48.21%) and D. stramonium (47.42%), whereas the lowest inhibition (37.52%) of mycelial growth was recorded at 5% leaf extract of A. indica as compared to control
In the present investigation antifungal activity of five plants extracts viz., E. globulus, C. procera, M. azedarach, D. stramonium and A. indica was assesed at the rate of 5%, 10% and 20% concentration against the mycelial growth and sporulation of A. alternata by poison food technique
Summary
Barley is an important rabi season crop of India and plays a major role in barley production all over the world. Ranking of barley is next to the maize, wheat and rice both in acreage and in production of grain. It is very nutritious and a rich source of Vitamin ‘B’ complex and Protein. The majority of the diseases are caused by seed-borne fungi. These seed-borne pathogens are resulting into losses or death of crop plants. The damage caused by plant parasitic pathogens and seed-borne fungi considered as worldwide and has extensive host range. Due to this they cause potentially serious constrains to crop productivity
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