Abstract
A salt-tolerant and alkaliphilic actinomycete BS30 isolated from a Saharan soil sample of Algeria, exhibited optimum growth at 2 % (w/v) NaCl and pH10. The strain exhibited antimicrobial activity against yeast and toxinogenic fungi. Analysis of the 16S rRNA gene sequence (1476 nucleotides) of the BS30 strain showed 98.77 % similarity with 16S rRNA gene sequence of Streptomyces spiroverticillatus NBRC 12821T, the most closely related type species. The phylogenetic analysis indicated that the BS30 strain represent a distinct phyletic line suggesting a potential new genomic species. Two bioactive molecules were isolated from the culture broth using various separation and purification steps. The first partially purified bioactive product was subjected to gas chromatography–mass spectrometry (GC–MS). The second fraction was analyzed by ultraviolet–visible (UV–VIS) spectrometry, electro-spray ionization mass (ESI–MS) and high resolution mass spectrometry (HR-ESI–MS) coupled with analytical liquid chromatography (HPLC) with a diode array detector (DAD). The two compounds showed significant antifungal activity against Aspergillus niger 2CA936.
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Schedule a callMore From: Proceedings of the National Academy of Sciences, India Section B: Biological Sciences
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