ANTIFUNGAL EFFECTS OF TAPINANTHUS GLOBIFERUS GROWING ON VITEXDONIANA AGAINST SOME FUNGAL ISOLATES

Abstract

Objective: Fungal infections are the major cause of many skin diseases, especially in developing countries. Natural products of medicinal value represent a potential source of chemotherapeutic agents. Tapinanthus globiferus has been used extensively in ethnomedicine for the treatment hypertension, ulcer, cancer, diabetes and fungal infections without a scientific basis. This work was aimed at screening the phytochemical constituents and evaluating the antifungal properties of methanol leaf extract its ethyl acetate and n-butanol fractions of T. globiferus against some clinical fungal isolates including Candida albicans, Trychophyton mentagrophytes, Trychophyton rubrum and Aspergillus niger using agar well diffusion and broth micro-dilution techniques.
 Methods: Preliminary screening of phytochemical constituents of extract and fractions of T. globiferus indicated the presence of carbohydrates, alkaloids, glycosides, tannins, flavonoids, saponins, steroids and triterpenes.
 Results: The methanol extract and its fractions demonstrated significant (P<0.05) antifungal effect against all the test organisms with mean zone of inhibition ranging from 27.83±0.16 – 14.46±0.29mm which was higher compared to that of the standard drug, Fluconazole (26.1±0.44 –18.49±0.16 mm). The minimum inhibitory concentration (MIC) and minimum fungicidal concentration (MFC) of the extract ranged between 6.25 – 25.0 mg/mL; ethyl acetate fraction had 3.13 – 25.0 mg/mL while n-butanol fraction had the least MIC ranging from 0.39-12.5 mg/mL against the test organisms.
 Conclusion: Study concluded that T. globiferus have good antifungal activity validating the ethnomedicinal claim for the use of the plant in the treating fungal diseases.
 Peer Review History: 
 Received 2 January 2020; Revised 25 February; Accepted 1 March, Available online 15 March 2020
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 Received file 
 
 Average Peer review marks at initial stage: 6.0/10
 Average Peer review marks at publication stage: 7.5/10
 Reviewer(s) detail:
 Name: Dr. Gehan Fawzy Abdel Raoof Kandeel
 Affiliation: Pharmacognosy Department, National Research Centre, Dokki, 12622, Giza, Egypt
 E-mail: gehankandeel9@yahoo.com
 
 Name: Dr. Ali Gamal Ahmed Al-kaf
 Affiliation: Sana'a university, Yemen
 E-mail: alialkaf21@gmail.com
 
 Name: Dr. Mahmut Yıldıztekin
 Affiliation: Muğla Sıtkı Koçman University, Turkey
 E-mail: mahmutyildiztekin@mu.edu.tr
 Comments of reviewer(s): 
 
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