Antifungal effect of 7-hydroxycitronellal against C. Tropicalis strains: an in vitro approach

Abrahão Alves Oliveira Filho,Cássio Ilan Soares Medeiros,Heloísa Mara Batista Fernandes De Oliveira,Hilzeth De Luna Freire Pessôa,José Pinto De Siqueira Júnior,Edeltrudes De Oliveira Lima

doi:10.14393/bj-v33n1a2017-33851

Abrahão Alves Oliveira Filho, Cássio Ilan Soares Medeiros + Show 4 more

Open Access

https://doi.org/10.14393/bj-v33n1a2017-33851

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Abstract

The fungi of the genus Candida play a relevant role in the emergence of oral infections, and are increasingly more frequent the cases of infections by non- albicans strains. In light of this context and the need for new alternatives to the antimicrobial therapy, the monoterpene [7-hidroxicitronelal] (7-HO) was evaluated for its antifungal effects. For the obtainment of the MIC and MFC values the broth microdilution method was used. The MICand the MFC of this monoterpene for 60% of the tested strains was of 256µg/mL and 512µg/mL respectively. Furthermore the standard antifungal Nystatin (100 UI/mL) was used as positive control for the inhibition of fungal growth. Therefore, were used 4 clinical strains of the species tropicalis (LM 06, LM 14, LM 31 and LM 36) and a standard strain ( C. tropicalis ATCC 13803), originated from the Mycology collection of the Mycology Laboratory (LM) of the Health Sciences Center (CCS) of the Federal University of Paraiba (UFPB). The results obtained in this study showed fungicide activity of the compound (7-OH) against the strains of Candida tropicalis .

Highlights

Oral candidiasis has been defined as an infection caused by fungi present in the microbiota of the oral cavity, Candida species are the most related to the infectious disorder
C. albicans is the most frequently species isolated as a colonizer pathogen of the oral mucosa, other species of Candida, such as C. tropicalis, C. krusei, C. glabrata, C. dubliniensis, C. guilliermondii, C. parapsilosis, C. kefyr, and C. pelliculosa, have become an importante cause of infection, mainly in immunocompromised patients and those affected by HIV (NWEZE, OGBONNAYA, 2011)
The results of the antifungal activity, minimum inhibitory concentration (MIC) and minimum fungicidal concentration (MFC) of the 7-OH for the strains of C. tropicalis are shown in the (Table 1 and 2), respectively

Summary

Introduction

Oral candidiasis has been defined as an infection caused by fungi present in the microbiota of the oral cavity, Candida species are the most related to the infectious disorder. C. albicans is the most frequently species isolated as a colonizer pathogen of the oral mucosa, other species of Candida, such as C. tropicalis, C. krusei, C. glabrata, C. dubliniensis, C. guilliermondii, C. parapsilosis, C. kefyr, and C. pelliculosa, have become an importante cause of infection, mainly in immunocompromised patients and those affected by HIV (NWEZE, OGBONNAYA, 2011). Due to the occurrence of undesirable factors such as the emergence of resistance to conventional antifungal drugs on the part of some strains especially in immunocompromised individuals and in general the presence of toxic effects (ZHAO et al, 2016), the study of natural products from plants with therapeutic properties, including those with antimicrobial activity has grown a lot, aiming to establish itself as an alternative therapeutic resource, and due to the perspectives of isolating substances with significant efficiency and lower disadvantage rates (MISHRA et al, 2016; CHINSEMBU, 2016).

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