Antifungal diterpenoids of Pseudolarix kaempferi, and their structure–activity relationship study

Abstract

The in vitro antifungal activities of 19 structurally diversified analogues of pseudolaric acids tested against the major pathogenic fungus Candida albicans has led to the establishment of a very clear structure–activity relationship of pseudolaric acids derivatives. Pseudolaric acid A was first found to be a potent antifungal component comparable with pseudolaric acid B. Among the tested 19 diterpenoids, pseudolaric acids A 2 ( 1), B 2 ( 3), B 3 ( 4) and methyl pseudolarate A 2 ( 2) are new isolates of the root bark of Pseudolarix kaempferi, and their structures were elucidated mainly by 2D-NMR techniques and chemical methods. Compounds 12– 19 were first semi-synthesized by efficient routines from pseudolaric acid B.