Abstract
Dried ground aerial parts were extracted successively with dichloromethane and methanol at room temperature. Antifungal activity was determined by a TLC bioassay using Cladosporium cucumerinum. Four compounds were isolated from a dichloromethane extract by different chromatographic techniques (silicagel CC, Sephadex LH-20 gel filtration, and low-pressure LC on Diol): the isoflavones irilin A and irilin B, the flavonone dihydrowogonin, and sesquiterpene pygmol. The latter three were antifungal. The minimal amount that inhibited the fungal growth of C. cucumerinum was 5 μ for these compounds. Four flavonol glycosides were obtained from the non-fungicidal extract by Sephadex LH-20 gel filtration and centrifugal partition chromatography. The structures of the compounds were elucidated by spectroscopic methods (UV, 1H NMR, 13CNMR, MS).
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