Abstract
Ashwagandha [Withania somnifera (L.) Dunal] is an important medicinal plant and its medicinal properties have been attributed to various classes of withanolide compounds. In the present study, we evaluated antifungal and antibacterial activities of withanolide extracted from roots of ashwagandha, against five pathogenic, fungi and bacteria. The minimal inhibitory concentration (MIC) of withanolides against two fungi, F. oxysporum and A. brassica were observed to be 4826.25 and 4474.22 ppm respectively. Antibacterial activity of withanolide extract was tested against three bacteria, E. coli¸ Pseudomonas solanacearum and Pseudomonas vulgaris. The maximum zone of inhibition at 4000 ppm of withanolide extract against E. coli, P. solanacearum and P.vulgaris were observed to be 8.7mm, 12.1mm and 12.5mm respectively. Thus withanolides extract was found to be the inhibitor of pathogenic fungi and bacteria.
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