Antifungal activity of Salvia verticillata subsp. verticillata against fungal pathogens

Abstract

Aim: It was aimed to investigate the antifungal activity of the individual as well as the combine extract (leaf+root) of Salvia verticillata subsp. verticillata L. from Lamiaceae family which has ethnobotanical use. Material and Methods: The plant samples were collected during to flowering stage in June, Samandere village around, Duzce, Turkey. The collected plants were dried according to herbarium techniques. The powdered plant material was extraction using ethyl alcohol (96%). Antifungal effects of the extracts was evaluated for yeasts (Candida krusei ATCC 20298, C. parapsilosis ATCC 90112, C. guilliermondii ATCC 6260, C. albicans ATCC 10231, C. tropicalis ATCC 13808, C. glabrata ATCC 2001, Cryptococcus laurentii ATCC 34142, C. neoformans ATCC 90112, Geotrichum capitatum ATCC 28576, Debaryomyces hansenii DSM 70238, Rhodotorula rubra DSM 70403, and Kluyveromyces fragilis ATCC 8608) by visual broth microdilution method. Antifungal antibiotic ketoconazole was used in the study to compare the results. Results: MIC values ranged from 1.56 to 25.00 mg/mL. The extracts have shown potential antifungal effects against the yeast cultures. Notably, the combine plant extracts (both leaves and rootstock) possessed higher antifungal potency against C. neoformans, C. laurentii and G. capitatum. Conclusion: Our results demonstrated that the extracts of Salvia verticillata subsp. verticillata are used for the treatment of fungal infections as traditional medicine