Antifungal activity of plant extracts onPhaeomoniella chlamydospora

Carine Rusin,Renato Vasconcellos Botelho,Marcus André Kurtz Almança,Cacilda Márcia Duarte Rios Faria,Carla Daiane Leite,Ires Cristina Ribeiro Oliari,Jean-Marie Aurand

doi:10.1051/bioconf/20160701044

Abstract

The decline and death of the vine has become an obstacle to world wine production. Among the causative agents highlights the Phaeomoniella chlamydospora , causal agent of Esca and Petri disease. Plant extracts may become a viable option control considering their fungistatic and/or fungicide substances. The objective of this work was to verify the potential of the aqueous extracts of plants on the control of Phaeomoniella chlamydospora comparing to other products. The following treatments were applied: sulfur, mancozeb, difeconazole, pyraclostrobin, tebuconazole, chitosan, Bacillus subtilis, Trichoderma harzianum, and extracts of india flowers, cinnamon bark, dried leaves of rosemary and lemon grass. The treatments were added in PDA culture medium, previously autoclaved at 120o for 20 min. Disc of 5 mm diameter of P.chlamydospora colony were transferred to the center of Petri dishes and kept at 20∘ C in the dark. The experimental design was completely randomized with five replications. It was evaluated the mycelial growth at five, eight and fourteen days after the installation of the experiment, obtaining the area under curve of the mycelial growth (AUCMG). The clove India extracts, cinnamon and rosemary, proved to be a control option considering their effect in the decrease of AUCMG compared to the control.

Highlights

The Chlamydospora Phaeomoniella, one of the causative agents of Esca’s disease and Petri disease, along with several other stem funguses, has become an obstacle to world wine production
Fungi associated with the decline of the vine which is usually infected by pruning wounds and/or grafting, may lead to plant death
It can be manifested by a sudden defoliation, but usually starts with a slow decline that translates into a reduced growth and several other symptoms such as chlorosis, necrosis and withered leaves

Summary

Introduction

The Chlamydospora Phaeomoniella, one of the causative agents of Esca’s disease and Petri disease, along with several other stem funguses, has become an obstacle to world wine production. Fungi associated with the decline of the vine which is usually infected by pruning wounds and/or grafting, may lead to plant death. It can be manifested by a sudden defoliation, but usually starts with a slow decline that translates into a reduced growth and several other symptoms such as chlorosis, necrosis and withered leaves. Internal symptoms in adult plants can be characterized by white rot in the trunk, which the evolution can cause cracks. They can be observed black dots, and brown or black streaks in xylem tissues [2]

Objectives

Methods

Results